@article{102216,
  keywords = {Diterpenes, Trypanosoma cruzi, Clerodane, NCTC cells, Echinodorus grandiflorus},
  author = {Borges MM and Moriconi JPV and de Pinho AO and Londero VS and Tempone AG and Lago JHG and Ferreira EA},
  title = {Anti-Trypanosoma cruzi activity of clerodane diterpenes and their methyl ester derivatives from Aquarius grandiflorus (Alismataceae)},
  abstract = {<p>The effect of the EtOAc extract of&nbsp;<em>Aquarius grandiflorus</em>&nbsp;leaves against&nbsp;<em>Trypanosoma cruzi</em>&nbsp;(trypomastigotes) was evaluated&nbsp;<em>in vitro</em>. As this extract caused 100% parasite reduction (at 300 µg/mL), it was subjected to chromatographic fractionation to obtain two related diterpenes, clerodermic (<strong>1</strong>) and patagonic (<strong>2</strong>) acids. Both compounds showed antiparasitic potential against&nbsp;<em>T. cruzi</em>&nbsp;with EC<sub>50</sub>&nbsp;of 41.7 µM (trypo)/60.7 µM (ama) and 37.6 µM (trypo)/73.1 µM (ama), respectively. Considering that the antiparasitic activity of carboxylic acid diterpenoids may be enhanced after esterification, methylation of both compounds was performed. The EC<sub>50</sub>&nbsp;values for methyl esters&nbsp;<strong>1a</strong>&nbsp;and&nbsp;<strong>2a</strong>&nbsp;were determined to be 19.7 µM (trypo)/7.5 µM (ama) and 21.8 µM (trypo)/9.3 µM (ama), respectively. Based on the potency of the methyl esters, they may be future candidates for optimisation studies to design new drugs for treatment of Chagas’ disease.</p>
},
  year = {2025},
  journal = {Natural Product Research},
  pages = {1-6},
  publisher = {Informa UK Limited},
  issn = {1478-6419, 1478-6427},
  url = {https://www.tandfonline.com/doi/full/10.1080/14786419.2025.2500732},
  doi = {10.1080/14786419.2025.2500732},
  language = {eng},
}