@article{98018,
  keywords = {multidisciplinary},
  author = {Cherkaoui D and Mesquita SG and Huang D and Lugli EB and Webster BL and McKendry RA},
  title = {CRISPR-assisted test for Schistosoma haematobium},
  abstract = {<p>Schistosomiasis is a major neglected tropical disease targeted for elimination as a public health issue by 2030, however there is an urgent need for more sensitive and specific diagnostic tests suitable to resource-limited settings. Here we developed CATSH, a CRISPR-assisted diagnostic test for Schistosoma haematobium, utilising recombinase polymerase amplification, Cas12a-targeted cleavage and portable real-time fluorescence detection. CATSH showed high analytical sensitivity, consistent detection of a single parasitic egg and specificity for urogenital Schistosoma species. Thanks to a novel CRISPR-compatible sample preparation developed using simulated urine samples containing parasitic eggs, CATSH had a sample-to-result within 2&nbsp;h. The components of CATSH can be lyophilised, reducing cold chain dependence and widening access to lower and middle-income countries. This work presents a new application of CRISPR diagnostics for highly sensitive and specific detection of parasitic pathogens in remote areas and could have a significant impact on the elimination of neglected tropical diseases.</p>
},
  year = {2023},
  journal = {Scientific Reports},
  volume = {13},
  pages = {1-9},
  publisher = {Springer Science and Business Media LLC},
  issn = {2045-2322},
  url = {https://www.nature.com/articles/s41598-023-31238-y.pdf?pdf=button%20sticky},
  doi = {10.1038/s41598-023-31238-y},
  language = {Eng},
}