01935nas a2200265   4500000000100000008004100001260002300042653001500065653002200080653001400102653001500116653002900131100001400160700001700174700001600191700001500207700001500222700001300237700001600250245013700266856007100403300000800474520116200482022002501644       2025                            d  bInforma UK Limited10aDiterpenes10aTrypanosoma cruzi10aClerodane10aNCTC cells10aEchinodorus grandiflorus1 aBorges MM1 aMoriconi JPV1 ade Pinho AO1 aLondero VS1 aTempone AG1 aLago JHG1 aFerreira EA00aAnti-Trypanosoma cruzi activity of clerodane diterpenes and their methyl ester derivatives from Aquarius grandiflorus (Alismataceae)  uhttps://www.tandfonline.com/doi/full/10.1080/14786419.2025.2500732  a1-63 a<p>The effect of the EtOAc extract of&nbsp;<em>Aquarius grandiflorus</em>&nbsp;leaves against&nbsp;<em>Trypanosoma cruzi</em>&nbsp;(trypomastigotes) was evaluated&nbsp;<em>in vitro</em>. As this extract caused 100% parasite reduction (at 300 µg/mL), it was subjected to chromatographic fractionation to obtain two related diterpenes, clerodermic (<strong>1</strong>) and patagonic (<strong>2</strong>) acids. Both compounds showed antiparasitic potential against&nbsp;<em>T. cruzi</em>&nbsp;with EC<sub>50</sub>&nbsp;of 41.7 µM (trypo)/60.7 µM (ama) and 37.6 µM (trypo)/73.1 µM (ama), respectively. Considering that the antiparasitic activity of carboxylic acid diterpenoids may be enhanced after esterification, methylation of both compounds was performed. The EC<sub>50</sub>&nbsp;values for methyl esters&nbsp;<strong>1a</strong>&nbsp;and&nbsp;<strong>2a</strong>&nbsp;were determined to be 19.7 µM (trypo)/7.5 µM (ama) and 21.8 µM (trypo)/9.3 µM (ama), respectively. Based on the potency of the methyl esters, they may be future candidates for optimisation studies to design new drugs for treatment of Chagas’ disease.</p>
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