02194nas a2200337 4500000000100000008004100001260003300042653002400075653001900099653001300118653001000131653001300141100001600154700001300170700001300183700002000196700001200216700001200228700001500240700001200255700001600267700001600283700001200299700001100311245017600322856007100498300001200569490000600581520124400587022002501831 2025 d bFederal University Dutsin-Ma10aOnchocerca volvulus10aonchocerciasis10aBlackfly10aAwhum10atourists1 aChikezie CI1 aUzoka NB1 aDalla CV1 aMonday-Chukwu O1 aIyam OI1 aOnwe DA1 aMachina IB1 aEdeh GC1 aOnyebuchi F1 aMaduforo AS1 aAwam CO1 aAni NM00aASSESSMENT OF THE PRESENCE OF SIMULIUM SPP BLACKFLIES AND ONCHOCERCIASIS IN SELECTED COMMUNITIES AROUND AWHUM WATERFALLS, A TOURIST SITE IN UDI LGA OF ENUGU STATE, NIGERIA uhttps://fjs.fudutsinma.edu.ng/index.php/fjs/article/view/3651/2489 a362-3680 v93 aThe study is an assessment of the abundance of blackflies (Simulium spp.) and Onchocerciasis in selected communities around Awhum waterfalls, a tourist site in Udi LGA of Enugu State, Nigeria. For the entomological aspect of this work, traps were set to catch and collect black flies in and around the Awhum Monastery which does not only serve as a tourist site but also a religious pilgrimage ground. Serological screening using Standard diagnostics test kits for Onchocerciasis (SD-Bioline OV16 kits) was used to screen 125 participants, who were members of the communities ranging from ages 5 – 70 years and comprising of 50 males and 75 females. The result revealed that only 28 (22.4%) of the participants were positive while majority 97(77.60%) were negative. Nineteen (19) positive cases were confirmed by molecular analysis of mitochondrial cytochrome oxidase subunit I (COI) gene. Of the total positive cases, more males 16(57.1 4%) than females 12(42.86%) were infected with Oncocerca volvulus. The findings confirmed the presence of blackflies and the endemicity of Onchocerciasis (River Blindness) in communities around the Awhum waterfalls, a tourist site in Awhum, Udi Local Government Area of Enugu State. a2616-1370, 2645-2944