02547nas a2200373   4500000000100000008004100001260001200042653001700054653001400071653002200085653001300107100001600120700001500136700001300151700001700164700001500181700001400196700001600210700001300226700001500239700001500254700001600269700001700285700001400302700001800316700001400334700002100348245016800369856006700537300000900604490000700613520153900620022001402159       2025                            d  bMDPI AG10aEpidemiology10aInfection10aTrypanosoma cruzi10aZoonosis1 aPacheco JIM1 aKmetiuk LB1 aFarias M1 aGonçalves G1 aFreitas AR1 aBiondo LM1 ade Paula CA1 aDelai RR1 aPimpão CT1 aPerotta JH1 aGiuffrida R1 aSantarém VA1 aLangoni H1 aFigueiredo FB1 aBiondo AW1 aBarros Filho IRD00aOne Health Approach to Trypanosoma cruzi: Serological and Molecular Detection in Owners and Dogs Living on Oceanic Islands and Seashore Mainland of Southern Brazil  uhttps://www.mdpi.com/2414-6366/10/8/220/pdf?version=1754138532  a1-110 v103 a<p>Via a One Health approach, this study concomitantly assessed the susceptibility of humans and dogs to Trypanosoma cruzi infections on three islands and in two mainland seashore areas of southern Brazil. Human serum samples were tested using an enzyme-linked immunosorbent assay (ELISA) to detect anti-T. cruzi antibodies, while dog serum samples were tested using indirect fluorescent antibodies in an immunofluorescence assay (IFA). Seropositive human and dog individuals were also tested using quantitative polymerase chain reaction (qPCR) in corresponding blood samples. Overall, 2/304 (0.6%) human and 1/292 dog samples tested seropositive for T. cruzi by ELISA and IFA, respectively, and these cases were also molecularly positive for T. cruzi by qPCR. Although a relatively low positivity rate was observed herein, these cases were likely autochthonous, and the individuals may have been infected as a consequence of isolated events of disturbance in the natural peridomicile areas nearby. Such a disturbance could come in the form of a fire or deforestation event, which can cause stress and parasitemia in wild reservoirs and, consequently, lead to positive triatomines. In conclusion, T. cruzi monitoring should always be conducted in suspicious areas to ensure a Chagas disease-free status over time. Further studies should also consider entomological and wildlife surveillance to fully capture the transmission and spread of T. cruzi on islands and in seashore mainland areas of Brazil and other endemic countries.</p>
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