02533nas a2200385   4500000000100000008004100001260001300042653002400055653001200079653002600091653002400117653001900141653003800160653003200198653002900230653001100259653002800270653001300298653003200311653002000343653002200363100001600385700001700401700001800418700001600436700001400452700001500466700001300481245011600494856007800610300001000688490000700698520142800705022001402133       1994                            d  c1994 Apr10aAmino Acid Sequence10aAnimals10aAntibodies, Protozoan10aAntigens, Protozoan10aChagas disease10aEnzyme-Linked Immunosorbent Assay10aEvaluation Studies as Topic10aFalse Positive Reactions10aHumans10aMolecular Sequence Data10aPeptides10aSensitivity and Specificity10aSerologic Tests10aTrypanosoma cruzi1 aPeralta J M1 aTeixeira M G1 aShreffler W G1 aPereira J B1 aBurns J M1 aSleath P R1 aReed S G00aSerodiagnosis of Chagas' disease by enzyme-linked immunosorbent assay using two synthetic peptides as antigens.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC267164/pdf/jcm00004-0127.pdf  a971-40 v323 a<p>An enzyme-linked immunosorbent assay (ELISA) was developed for detecting antibodies against Trypanosoma cruzi. Two synthetic T. cruzi peptides, TcD and PEP2, were used. The specificity and sensitivity of the peptide ELISA were determined with 260 serum samples from individuals living in an area in which Chagas' disease is endemic. ELISAs were performed with the peptides singly or in combination. The evaluation of these tests showed that 168 (93.8%) of 179 serum samples from T. cruzi-infected patients were positive when TcD peptide was used as antigen; 164 (91.6%) samples were positive with PEP2, and 178 (99.4%) samples were positive when the two peptides were combined. Thus, the sensitivity of the ELISA using the two peptides exceeded 99%. The specificity was evaluated by using a panel of 118 serum samples that included samples from 81 individuals living in an area of endemicity with negative serology for Chagas' disease and from 37 patients from areas in which T. cruzi was not endemic but with other pathologies, such as leishmaniasis, tuberculosis, and leprosy. Only two false-positive serum samples were found in this group of individuals, giving a test specificity of more than 98%. Because these peptides can be synthesized and are very stable at room temperature, the use of such reagents can improve the standardization and reproducibility of ELISAs for the serodiagnosis of T. cruzi infection.</p>  a0095-1137