01285nas a2200157   4500000000100000008004100001653011500042100001200157700001200169700001500181245017400196300001100370490000800381520072400389022001401113       2015                            d10aDrug Susceptibility Profiling; Genetic Biomarker; Gold Nanoparticle; Polymeric Chain Reaction; Visual Dtection1 aBose PP1 aKumar P1 aMunagala N00aConcurrent visual diagnosis and susceptibility profiling of the first line drug against visceral leishmaniasis by plasmonic detection of PCR amplified genetic biomarker.  a208-140 v1523 a<p>Visceral form of Leishmaniasis (also known as Kala-azar) is a fatal neglected tropical disease affecting 95 countries worldwide. Recently, substantial proportion of resistance related treatment failure cases have been reported against its first line drug, Sodium-antimony gluconate (SAG). We report an easy, fast, sensitive and cheap visual diagnosis and SAG susceptibility profiling for this disease based on recently recognized genetic biomarker and gold nanoparticle based plasmonic detection phenomenon. This is a non-gel, non-culture based detection technique, which can be used as simultaneous high throughput detection and SAG-susceptibility profiling in Leishmania endemic resource stringent countries.</p>
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