01832nas a2200169   4500000000100000008004100001653002200042653001600064100001500080700001400095245010300109856009000212300001300302490000700315520132600322022001401648       2016                            d10aGenomic databases10aEbola virus1 aCarneiro J1 aPereira F00aEbolaID: an online database of informative genomic regions for ebola identification and treatment.  uhttp://journals.plos.org/plosntds/article/asset?id=10.1371%2Fjournal.pntd.0004757.PDF  ae00047570 v103 a<p>The Ebola virus disease (EVD) is a rare and deadly disease affecting humans and other primates caused by infection with a virus of the family Filoviridae, genus Ebolavirus. The March 2014 Ebola epidemic is the largest in history, affecting multiple countries in West Africa, with more than 11,301 deaths reported by the World Health Organization by March 2016. Methods based on polymerase chain reaction (PCR) are commonly used for virus detection with the advantage of requiring low amounts of viral samples, minimal manipulation, and minimal equipment. In particular, quantitative PCR (qPCR) has been used for the sensitive and fast identification of patients with EVD. However, the high genetic diversity of RNA viruses poses a challenge for the design of efficient nucleic acid-based assays, as suggested by the high substitution rate observed in Ebola virus from the 2014 outbreak. Recent studies suggest that false-negative diagnosis or inefficient therapeutics can occur due to sequence variation at binding sites of PCR primers, probes, small interfering RNAs (siRNAs), or monoclonal antibodies targeting the Ebola virus. For this reason, the selection of reliable oligonucleotides and target genomic regions for use in nucleic acid-based assays is crucial for the correct diagnosis and treatment of EVD.</p>
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