02506nas a2200229   4500000000100000008004100001260001300042653001500055653001400070100001400084700001600098700001300114700001400127700001300141700001100154700001100165700001400176700001300190700001800203245007900221520197600300       2016                            d  bElsevier10aWound care10aTreatment1 aBaliano A1 aPimentel EF1 aBuzin AR1 aVieira TZ1 aRomão W1 aTose L1 aLenz D1 aAndrade T1 aFronza M1 aKondratyuk TP00aBrown seaweed Padina gymnospora is a prominent natural wound-care product.3 a<p>Seaweeds are related to anti-inflammatory, anti-bacterial and anti-noceptive effects. This work aimed to verify the potential of seaweed Padina gymnospora (Kützing) Sonder 1871 to improve wound healing in vitro. P. gymnospora was collected at a bethonic area in Espirito Santo. Methanolic extract of P. gymnospora was obtained by percolation. To determine cytotoxicity, colorimetric MTT tests were performed against normal fibroblasts (L929), macrophages (RAW 264.7) and human ovarian carcinoma (OVCAR-3) cell lines using concentration range of 12–110&nbsp;μg&nbsp;ml−1. To evaluate in vitro wound healing, monolayer of fibroblasts L929 was seeded and artificial wounded. Cell proliferation was blocked by 5&nbsp;μg&nbsp;ml−1 Mytomycin C. Nitric oxide inhibition was quantified with Raw 264.7 by Griess reaction. Minimal inhibitory concentration (MIC) against Staphylococcus aureus was determined. Eletrospray ionization with Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS) was applied to detail composition of P. gymnospora methanolic extract. No cytotoxic effect in all cell lines was detected until the maximum concentration of 110&nbsp;μg&nbsp;ml−1. P. gymnospora promoted significantly migration at the concentration of 25&nbsp;μg&nbsp;ml−1 (p&nbsp;&lt;&nbsp;0.05). A prominent inhibition of nitric oxide formation was achieved in a concentration of 20&nbsp;μg&nbsp;ml−1 of methanolic extract of P. gymnospora (62.06&nbsp;±&nbsp;1.20%). Antibacterial activity against S. aureus could be demonstrated with MIC of 500&nbsp;μg&nbsp;ml−1. ESI-FT-ICR MS analysis indicated eleven molecules between then, linolenic, oleic and linoleic acid. P. gymnospora favored wound repair in vitro what could be related to its fatty acid composition. In addition, its antimicrobial effect, and NO inhibition activity contribute for a new approach of P. gymnospora as a promise natural product for treatment of cutaneous wound.</p>