01552nas a2200241 4500000000100000008004100001653000900042653002600051653002900077653002300106653002700129100001300156700001700169700001600186700001700202700001700219700001500236245011700251300001200368490000800380520090800388022001401296 2017 d10aNTDs10aLutzomyia longipalpis10aMicropygomyia quinquefer10aNyssomyia whitmani10aVisceral Leishmaniasis1 aMoya S L1 aGiuliani M G1 aSantini M S1 aQuintana M G1 aSalomón O D1 aLiotta D J00aLeishmania infantum DNA detected in phlebotomine species from Puerto Iguazú City, Misiones province, Argentina. a122-1240 v1723 a

In Puerto Iguazú City, Argentina, human and canine Visceral Leishmaniasis cases have been recorded since the year 2010, with Leishmania infantum as the etiological agent and Lutzomyia longipalpis as its main vector. In the present study, polymerase chain reaction and sequencing were used to detect L. infantum DNA in 3.9% of the female sandflies captured in Puerto Iguazú City. This is the first report of L. infantum DNA detection in Micropygomyia quinquefer, and the second one in Lu. longipalpis and Nyssomyia whitmani for Argentina. Although the detection of Leishmania DNA itself is not enough to determine a Phlebotomine species as a vector, these results are significant in setting the direction of further investigations of vectorial competence and capacity, necessary to define the roles of different sandflies species as specific or permissive vectors in the transmission VL cycle.

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