01700nas a2200217   4500000000100000008004100001260004400042653002200086100001600108700001600124700001200140700001300152700001500165700001600180245005300196856007900249300000800328490000700336520112500343022001401468       2023                            d  bSpringer Science and Business Media LLC10amultidisciplinary1 aCherkaoui D1 aMesquita SG1 aHuang D1 aLugli EB1 aWebster BL1 aMcKendry RA00aCRISPR-assisted test for Schistosoma haematobium  uhttps://www.nature.com/articles/s41598-023-31238-y.pdf?pdf=button%20sticky  a1-90 v133 a<p>Schistosomiasis is a major neglected tropical disease targeted for elimination as a public health issue by 2030, however there is an urgent need for more sensitive and specific diagnostic tests suitable to resource-limited settings. Here we developed CATSH, a CRISPR-assisted diagnostic test for Schistosoma haematobium, utilising recombinase polymerase amplification, Cas12a-targeted cleavage and portable real-time fluorescence detection. CATSH showed high analytical sensitivity, consistent detection of a single parasitic egg and specificity for urogenital Schistosoma species. Thanks to a novel CRISPR-compatible sample preparation developed using simulated urine samples containing parasitic eggs, CATSH had a sample-to-result within 2&nbsp;h. The components of CATSH can be lyophilised, reducing cold chain dependence and widening access to lower and middle-income countries. This work presents a new application of CRISPR diagnostics for highly sensitive and specific detection of parasitic pathogens in remote areas and could have a significant impact on the elimination of neglected tropical diseases.</p>
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