TY - JOUR
KW - Enzyme-Linked Immunosorbent Assay
KW - HSP70 Heat-Shock Proteins
KW - Humans
KW - Leishmania
KW - Leishmaniasis
KW - Oligonucleotides
KW - Protozoan Proteins
KW - ROC Curve
KW - Recombinant Proteins
KW - Serologic Tests
KW - Statistics, Nonparametric
AU - Souza AP
AU - Soto M
AU - Costa J
AU - Boaventura V
AU - Oliveira CI
AU - Cristal JR
AU - Barral-Netto M
AU - Barral A
AB - <p><b>BACKGROUND: </b>Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure.</p><p><b>METHODOLOGY/PRINCIPAL FINDINGS: </b>Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis (TL) against different L. infantum-chagasi-derived recombinant proteins (rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11). Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas' disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA.</p><p><b>CONCLUSION: </b>Our results raise possibility of using rHSP70 for the serodiagnosis of TL.</p>
BT - PloS one
C1 - http://www.ncbi.nlm.nih.gov/pubmed/23776617?dopt=Abstract
DA - 2013
DO - 10.1371/journal.pone.0066110
IS - 6
J2 - PLoS ONE
LA - eng
N2 - <p><b>BACKGROUND: </b>Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure.</p><p><b>METHODOLOGY/PRINCIPAL FINDINGS: </b>Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis (TL) against different L. infantum-chagasi-derived recombinant proteins (rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11). Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas' disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA.</p><p><b>CONCLUSION: </b>Our results raise possibility of using rHSP70 for the serodiagnosis of TL.</p>
PY - 2013
EP - e66110
T2 - PloS one
TI - Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins.
UR - http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0066110&representation=PDF
VL - 8
SN - 1932-6203
ER -