TY - JOUR KW - Rifampicin KW - Buruli ulcer AU - Jansson M AU - Beissner M AU - Phillips RO AU - Badziklou K AU - Piten E AU - Maman I AU - Sarfo FS AU - Huber KL AU - Rhomberg A AU - Symank D AU - Wagner M AU - Wiedemann FX AU - Nitschke J AU - Kere AB AU - Herbinger K AU - Adjei O AU - Löscher T AU - Bretzel G AB -

This study evaluates a novel assay to detect rifampicin resistance in clinical Mycobacterium ulcerans isolates. Although highly susceptible for PCR inhibitors in 50% of samples tested, the assay was 100% M. ulcerans-specific and yielded >98% analyzable sequences with a lower limit of detection of 100-200 copies of the target sequence.

BT - Journal of clinical microbiology C1 -

http://www.ncbi.nlm.nih.gov/pubmed/24478404?dopt=Abstract

DA - 2014 Jan 29 DO - 10.1128/JCM.03119-13 J2 - J. Clin. Microbiol. LA - eng N2 -

This study evaluates a novel assay to detect rifampicin resistance in clinical Mycobacterium ulcerans isolates. Although highly susceptible for PCR inhibitors in 50% of samples tested, the assay was 100% M. ulcerans-specific and yielded >98% analyzable sequences with a lower limit of detection of 100-200 copies of the target sequence.

PY - 2014 T2 - Journal of clinical microbiology TI - Molecular determination of Rifampicin resistance in clinical samples of patients with buruli ulcer disease: A comparison of two assays. UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993512/pdf/zjm1246.pdf SN - 1098-660X ER -