TY - JOUR
KW - General Physics and Astronomy
KW - General Biochemistry, Genetics and Molecular Biology
KW - General Chemistry
AU - Berger DJ
AU - Crellen T
AU - Lamberton PHL
AU - Allan F
AU - Tracey A
AU - Noonan JD
AU - Kabatereine NB
AU - Tukahebwa EM
AU - Adriko M
AU - Holroyd N
AU - Webster JP
AU - Berriman M
AU - Cotton JA
AB - <jats:title>Abstract</jats:title><jats:p>Control and elimination of the parasitic disease schistosomiasis relies on mass administration of praziquantel. Whilst these programmes reduce infection prevalence and intensity, their impact on parasite transmission and evolution is poorly understood. Here we examine the genomic impact of repeated mass drug administration on <jats:italic>Schistosoma mansoni</jats:italic> populations with documented reduced praziquantel efficacy. We sequenced whole-genomes of 198 <jats:italic>S. mansoni</jats:italic> larvae from 34 Ugandan children from regions with contrasting praziquantel exposure. Parasites infecting children from Lake Victoria, a transmission hotspot, form a diverse panmictic population. A single round of treatment did not reduce this diversity with no apparent population contraction caused by long-term praziquantel use. We find evidence of positive selection acting on members of gene families previously implicated in praziquantel action, but detect no high frequency functionally impactful variants. As efforts to eliminate schistosomiasis intensify, our study provides a foundation for genomic surveillance of this major human parasite.</jats:p>
BT - Nature Communications
DO - 10.1038/s41467-021-24958-0
IS - 1
N2 - <jats:title>Abstract</jats:title><jats:p>Control and elimination of the parasitic disease schistosomiasis relies on mass administration of praziquantel. Whilst these programmes reduce infection prevalence and intensity, their impact on parasite transmission and evolution is poorly understood. Here we examine the genomic impact of repeated mass drug administration on <jats:italic>Schistosoma mansoni</jats:italic> populations with documented reduced praziquantel efficacy. We sequenced whole-genomes of 198 <jats:italic>S. mansoni</jats:italic> larvae from 34 Ugandan children from regions with contrasting praziquantel exposure. Parasites infecting children from Lake Victoria, a transmission hotspot, form a diverse panmictic population. A single round of treatment did not reduce this diversity with no apparent population contraction caused by long-term praziquantel use. We find evidence of positive selection acting on members of gene families previously implicated in praziquantel action, but detect no high frequency functionally impactful variants. As efforts to eliminate schistosomiasis intensify, our study provides a foundation for genomic surveillance of this major human parasite.</jats:p>
PB - Springer Science and Business Media LLC
PY - 2021
T2 - Nature Communications
TI - Whole-genome sequencing of Schistosoma mansoni reveals extensive diversity with limited selection despite mass drug administration
VL - 12
SN - 2041-1723
ER -