TY - JOUR
KW - Agglutination Tests
KW - Antibodies
KW - Antigens, Protozoan
KW - Chagas disease
KW - Cross Reactions
KW - Humans
KW - Leishmania donovani
KW - Mercaptoethanol
KW - Rosaniline Dyes
KW - Staining and Labeling
KW - Trypanosoma cruzi
KW - Trypsin
AU - Harith A
AU - Laarman J J
AU - Minter-Goedbloed E
AU - Kager P A
AU - Kolk A H
AB - <p>A microagglutination test using trypsin-treated and Coomassie blue-stained Trypanosoma cruzi epimastigote antigen was adapted for the diagnosis of Chagas' disease. When incorporated in the test, 2-mercaptoethanol treatment of chagasic sera had no influence on antibody titer. In contrast, titers in sera from patients with visceral leishmaniasis, African trypanosomiasis, and autoimmune disorders, subjected to similar treatment, showed remarkable decline. Accordingly, a lower cut-off point for Chagas' disease serological negativity could be taken resulting in a higher sensitivity (95.6%); the specificity was 94.7%. Similar specificities were obtained with Leishmania donovani chagasi and L. d. donovani antigens applied to homologous visceral leishmaniasis and heterologous Chagas' sera. Of 316 nonchagasic sera, only 3 with leptospirosis and 1 with leprosy showed seropositive titers prior to and after 2-mercaptoethanol treatment.</p>
BT - The American journal of tropical medicine and hygiene
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2440329?dopt=Abstract
DA - 1987 Jul
DO - 10.4269/ajtmh.1987.37.66
IS - 1
J2 - Am. J. Trop. Med. Hyg.
LA - eng
N2 - <p>A microagglutination test using trypsin-treated and Coomassie blue-stained Trypanosoma cruzi epimastigote antigen was adapted for the diagnosis of Chagas' disease. When incorporated in the test, 2-mercaptoethanol treatment of chagasic sera had no influence on antibody titer. In contrast, titers in sera from patients with visceral leishmaniasis, African trypanosomiasis, and autoimmune disorders, subjected to similar treatment, showed remarkable decline. Accordingly, a lower cut-off point for Chagas' disease serological negativity could be taken resulting in a higher sensitivity (95.6%); the specificity was 94.7%. Similar specificities were obtained with Leishmania donovani chagasi and L. d. donovani antigens applied to homologous visceral leishmaniasis and heterologous Chagas' sera. Of 316 nonchagasic sera, only 3 with leptospirosis and 1 with leprosy showed seropositive titers prior to and after 2-mercaptoethanol treatment.</p>
PY - 1987
SP - 66
EP - 71
T2 - The American journal of tropical medicine and hygiene
TI - Trypsin-treated and coomassie blue-stained epimastigote antigen in a microagglutination test for Chagas' disease.
VL - 37
SN - 0002-9637
ER -